CHIC Clone collection

Eggs from selected pathogen-free white Leghorn chickens were used as starting material for the production of epithelial chicken cell lines. Cells were isolated from the intestinal tract (intestine and cecum) of 18-day-old embryonic chickens and subsequently subjected to a specific procedure of growth factor treatment and limiting dilution cloning.

As result we succeeded in isolating more than 240 indigenous, continuously growing clones, which - according to microscopic classification - represent at least 5 different morphotypes. a number of 12 clones were further characterized with special regard to their epithelial character using biochemical and immunological methods. CHIC clone 8E11 is the most prominent representative.

Applications of the CHIC Clone cells

  • Infection and inhibition (susceptibility) tests to Campylobacter and Salmonella strains
  • Expression of recombinant proteins upon transfection / infection
  • Production of viral particles (e.g. influenza)


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If you are interested in a cell line, just write to us on our contact page. We will prepare an offer with controlled shipment of the product.


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  • Ali A, Kolenda R, Khan MM, Weinreich J, Li G, Wieler LH, Tedin K, Roggenbuck D, Schierack P. Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines. Appl Environ Microbiol. 2020 Oct 1;86(20):e01068-20. doi: 10.1128/AEM.01068-20. PMID: 32769194; PMCID: PMC7531953.
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  • Kim WH, Lillehoj HS, Min W. Indole Treatment Alleviates Intestinal Tissue Damage Induced by Chicken Coccidiosis Through Activation of the Aryl Hydrocarbon Receptor. Front Immunol. 2019 Mar 26;10:560. doi: 10.3389/fimmu.2019.00560. PMID: 30972060; PMCID: PMC6443889.
  • John DA, Williams LK, Kanamarlapudi V, Humphrey TJ, Wilkinson TS. The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells. Front Microbiol. 2017 Sep 29;8:1840. doi: 10.3389/fmicb.2017.01840. PMID: 29033908; PMCID: PMC5626877.
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