CHIC Clone collection

Eggs from selected pathogen-free white Leghorn chickens were used as starting mate­rial for the produc­tion of epithe­lial chicken cell lines. Cells were isolated from the intestinal tract (intes­tine and cecum) of 18-day-old embry­onic chickens and subse­quently subjected to a specific proce­dure of growth factor treat­ment and limiting dilu­tion cloning.

As result we succeeded in isolating more than 240 indige­nous, contin­u­ously growing clones, which – according to micro­scopic clas­si­fi­ca­tion – repre­sent at least 5 different morpho­types. a number of 12 clones were further char­ac­ter­ized with special regard to their epithe­lial char­acter using biochem­ical and immuno­log­ical methods. CHIC clone 8E11 is the most promi­nent representative.

Appli­ca­tions of the CHIC Clone cells

  • Infec­tion and inhi­bi­tion (suscep­ti­bility) tests to Campy­lobacter and Salmo­nella strains
  • Expres­sion of recom­bi­nant proteins upon trans­fec­tion / infection
  • Produc­tion of viral parti­cles (e.g. influenza)

Buy a cell line

If you are inter­ested in a cell line, just write to us on our contact page. We will prepare an offer with controlled ship­ment of the product.

Refer­ences

  • Khan MM, Kolenda R, Schierack P, Wein­reich J, Rödiger S, Schierack J, Stubbe M, Lkhag­va­suren D, Guen­ther S, Schau­fler K. Inves­ti­ga­tion of Commensal Escherichia coli Popu­la­tions of Cormorant Hatch­lings in the Absence of Anthro­pogenic Impacts in Remote Areas of West Mongolia. Microor­gan­isms. 2021 Feb 12;9(2):372. doi: 10.3390/microorganisms9020372. PMID: 33673351; PMCID: PMC7917637.
  • Ali A, Kolenda R, Khan MM, Wein­reich J, Li G, Wieler LH, Tedin K, Roggen­buck D, Schierack P. Novel Avian Path­o­genic Escherichia coli Genes Respon­sible for Adhe­sion to Chicken and Human Cell Lines. Appl Environ Micro­biol. 2020 Oct 1;86(20):e01068-20. doi: 10.1128/AEM.01068 – 20. PMID: 32769194; PMCID: PMC7531953.
  • Han X, Bertzbach LD, Veit M. Mimic­king the passage of avian influenza viruses through the gastroin­testinal tract of chickens. Vet Micro­biol. 2019 Dec;239:108462. doi: 10.1016/j.vetmic.2019.108462. Epub 2019 Oct 18. PMID: 31767100; PMCID: PMC7126190.
  • Pascoe B, Williams LK, Calland JK, Meric G, Hitch­ings MD, Dyer M, Ryder J, Shaw S, Lopes BS, Chin­toan-Uta C, Allan E, Vidal A, Fearnley C, Everest P, Pachebat JA, Cogan TA, Stevens MP, Humphrey TJ, Wilkinson TS, Cody AJ, Colles FM, Jolley KA, Maiden MCJ, Stra­chan N, Pearson BM, Linton D, Wren BW, Parkhill J, Kelly DJ, van Vliet AHM, Forbes KJ, Shep­pard SK. Domes­ti­ca­tion of Campy­lobacter jejuni NCTC 11168. Microb Genom. 2019 Jul;5(7):e000279. doi: 10.1099/mgen.0.000279. Epub 2019 Jul 16. PMID: 31310201; PMCID: PMC6700657.
  • Kim WH, Lillehoj HS, Min W. Indole Treat­ment Alle­vi­ates Intestinal Tissue Damage Induced by Chicken Coccidiosis Through Acti­va­tion of the Aryl Hydro­carbon Receptor. Front Immunol. 2019 Mar 26;10:560. doi: 10.3389/fimmu.2019.00560. PMID: 30972060; PMCID: PMC6443889.
  • John DA, Williams LK, Kana­mar­la­pudi V, Humphrey TJ, Wilkinson TS. The Bacte­rial Species Campy­lobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithe­lial Cells. Front Micro­biol. 2017 Sep 29;8:1840. doi: 10.3389/fmicb.2017.01840. PMID: 29033908; PMCID: PMC5626877.